some (but not all) purple photosynthetic bacteria. It is also found in the mitochondrion of all eukaryotic cells and is the pathway used by mitochondria to make ALA for heme biosynthesis.
The next series of biosynthetic steps lead to protoporphyrin IX. Step 4 is the condensation of two molecules of ALA to form the cyclic compound porphobilinogen, which represents the pyrrole monomer. Step 5 is the further condensation of four molecules of porphobilinogen to form the open‐chain tetrapyrrole hydroxymethylbilane. Steps 6–8 involve ring closure and successive oxidative decarboxylation steps. Note the remarkable “flipping” of pyrrole ring D during step 6, so that the positions of the acetate and propionate substituents are interchanged. This proceeds by way of a spiro intermediate at C‐16.
The final step in this phase of the biosynthetic pathway is the oxidation of protoporphyrinogen IX to protoporphyrin IX, step 9 in the sequence shown in Fig. 4.6. This is an important step, because the former compound looks superficially like a porphyrin, but is not fully conjugated. The pyrrole rings are essentially independent of each other, and the compound is colorless. The product, protoporphyrin IX, is a fully conjugated porphyrin and is highly colored. Excited protoporphyrin IX reacts readily with molecular oxygen to form the highly damaging species singlet oxygen. The system is highly regulated so that high concentrations of free protoporphyrin IX and other photosensitive intermediates do not build up.
Protoporphyrin IX is metallated by insertion of Mg at step 10. The heme biosynthesis pathway branches at this point (Bryant et al., 2020). Those molecules destined to become heme have Fe inserted instead of Mg. The next phase of the biosynthesis of chlorophylls involves the construction of the isocyclic ring E by cyclizing the propionic acid attached to C‐13. This reaction proceeds by first esterifying the carboxylic acid moiety and then undergoing a stereospecific oxidative cyclase reaction, steps 12–14. The intermediate at this step, divinyl protochlorophyllide, is then acted on by two separate enzymes on opposite sides of the ring. The two enzymes are not sensitive to whether the other change has been made in the substrate, so the pathway branches, and a given molecule can first have step 15 and then step 16 take place, or vice versa. Step 15 is the reduction of the vinyl group at C‐8 to an ethyl group, and step 16 is the reduction of ring D.
The reduction of ring D is one of the most interesting steps in the biosynthesis of chlorophylls. In all oxygenic photosynthetic organisms, a light‐driven enzyme, protochlorophyllide reductase, (often called POR), carries out this step. This enzyme uses NADPH as a source of reductant but also has an absolute requirement for light, making it one of a very small number of light‐driven enzymes known in all of biology (another is the DNA repair enzyme photolyase). The structure of the light‐driven POR from a cyanobacterium has been determined (Dong et al., 2020). In anoxygenic photosynthetic bacteria, an entirely different enzyme complex that is not light‐driven carries out this reduction. In this case, the reductant is reduced ferredoxin instead of NADPH. All oxygenic organisms except higher plants have both the light‐dependent and the light‐independent enzymes. The evolutionary implications of this pattern are discussed in Chapter 12.
The final step in chlorophyll a biosynthesis is the attachment of the tail, catalyzed by the enzyme chlorophyll synthase and using phytol pyrophosphate as the substrate. Phytol pyrophosphate is made by reduction of the unsaturated polyisoprenoid compound geranylgeranyl pyrophosphate. Depending on the state of growth of the organism, the reduction can take place either before or after attachment of the tail to the macrocycle.
The same basic pathway is used for the synthesis of all other chlorophylls and bacteriochlorophylls. Most of the reactions are the same, except that some steps are O2‐dependent in aerobes but use a different enzyme and oxidant in anaerobes (Ouchane et al., 2004; Raymond and Blankenship, 2004). The major differences are found at the end of the pathway. Bacteriochlorophyll a is made from chlorophyllide a using two additional steps. The first is the conversion of the C‐3 vinyl group to an acetyl group. The second is the reduction of pyrrole ring B by an enzyme complex similar to the light‐independent enzyme that reduces ring D, as discussed in the previous paragraph.
Figure 4.6 Outline of chlorophyll a biosynthesis from glutamate. The enzymes that catalyze the individual numbered reactions are (1) glutamyl‐tRNA synthetase; (2) glutamyl‐tRNA reductase; (3) glutamate 1‐semialdehyde aminotransferase; (4) porphobilinogen synthase; (5) hydroxymethylbilane synthase; (6) uroporphyrinogen III synthase; (7) uroporphyrinogen III decarboxylase; (8) coproporphyrinogen III oxidative decarboxylase; (9) protoporphyrinogen IX oxidase; (10) protoporphyrin IX Mg‐chelatase;(11) S‐adenosyl‐L‐methionine:Mg‐protoporphyrin IX methyl‐transferase; (12)–(14) Mg‐protoporphyrin IX monomethyl ester oxidative cyclase; (15) divinyl (proto)chlorophyllide 4‐vinyl reductase;(16) light‐dependent NADPH:protochlorophyllide oxidoreductase or light‐independent protochlorophyllide reductase; (17) chlorophyll synthase. The IUPAC numbering for the tetrapyrrole peripheral substituent positions is shown for protoporphyrin IX.
Source: Beale (1999) (p. 45)/Springer Nature.
Chlorophyll b is made from chlorophyll a by oxidation of the methyl group at C‐7 to give a formyl group, using a mixed‐function oxidase enzyme that depends on O2 (Tanaka and Tanaka, 2007). Chlorophyll b can also be reduced back to chlorophyll a. The ratio of the two pigments is tightly regulated and can be adjusted as needed.
4.4 Spectroscopic properties of chlorophylls
The chlorophylls all contain two major absorption bands, one in the blue or near UV region and one in the red or near IR region (Fig. 4.7). The blue absorption band produces a second excited state that very rapidly (picoseconds) loses energy as heat to produce the lowest excited state. The lowest excited state is relatively long‐lived (nanoseconds) and is the state that is used for electron transfer and energy storage in photosynthesis.
The lack of a significant absorption in the green region gives the chlorophylls their characteristic green or blue–green color. These absorption bands are π → π* transitions, involving the electrons in the conjugated π system of the chlorin macrocycle. The absorption and fluorescence spectra of chlorophyll a and bacteriochlorophyll a are shown in Fig. 4.8. The two lowest‐energy transitions are called the Q bands, and the two higher‐energy ones are known as the B bands. They are also commonly called Soret bands.
Figure 4.7 Absorption spectrum and simplified energy level diagram for chlorophyll a. The blue absorption band populates a second excited state that rapidly converts to the lowest excited state that can also be populated by absorption of a red photon. Energy storage in photosynthesis always occurs from the lowest excited state and the energy difference between the higher excited states and the lowest excited state is lost as heat.
Source: Blankenship et al. (2011) (p. 807)/American Association for the Advancement of Science.
The spectra can be described theoretically by using a “four orbital” model, originally proposed by Martin Gouterman (1961). The four π molecular orbitals that are principally involved in these transitions are the two highest occupied molecular
