does occur due to disputes or in situations in which animals are insured or involved in some form of competition.
Determining the cause and manner of death is critical to substantiating claims and the ultimate liability of insurers.
A systematic and thorough postmortem examination is essential to confirm death caused by toxicant exposure.
Documentation (e.g., use of chain‐of‐custody procedures) of proper sample collection, storage, and laboratory submission is crucial, especially with accidental feed contamination or malicious poisoning suspicions.
Toxicant testing can be targeted (i.e., testing for specific toxicants) or non‐targeted (i.e., looking for unknowns). While non‐targeted testing can identify many toxicants, there is no single comprehensive test for unknowns.
It is important to keep an open mind when investigating the death of any animal and not to be misled by allegations of malicious intent.
Exclusive of plants for which ingestion is associated with sudden death, potential toxicants include strychnine, phosphides, cholinesterase‐inhibiting insecticides (e.g., OPs, carbamates), nicotine, metaldehyde, cyanide, fluoroacetate, illicit drugs (e.g., amphetamines, cocaine, heroin, morphine), metals (e.g., mercury, arsenic, lead, selenium, iron), drugs (e.g., insulin, barbiturates, reserpine, succinylcholine), electrolytes (e.g., potassium, calcium) and vitamins A, D, and E.
Ingestion of toxic plants is a less common cause of malicious poisoning but still a possibility. Exposure to extremely toxic plants (e.g., Taxus spp. [yew], Nerium oleander [oleander], Conium maculatum [poison hemlock], Cicuta spp. [water hemlock]) should be considered, as should zootoxins (e.g., cantharidin).
Signs vary considerably depending on the specific toxicant to which a horse is exposed.
Most malicious intoxications are associated with administration of highly toxic drugs or chemicals intended to kill quickly.
An ideal toxicant used maliciously would cause rapid death, not result in specific postmortem lesions, and be difficult to detect in postmortem tissue or fluid samples. Fortunately, the list of toxicants meeting all three criteria is rather limited.
Most toxicants that result in sudden death impair the central or peripheral nervous systems, cardiovascular system, or respiratory system. Thus, if signs are noted before death, they generally relate to failure of one or more of these systems.
Intoxications can result in more chronic disease and multiple exposures might be required before onset of clinical signs (e.g., ingestion of pyrrolizidine alkaloid‐containing plants).
Depending on the toxicant, there might be evidence of struggle before death, as might occur following central nervous system (CNS) stimulation or respiratory impairment. Alternatively, some toxicant‐induced deaths are associated with no struggle before death, as might occur after administration of a barbiturate or other CNS depressant.
There are many causes of sudden or unexplained death other than toxicants:
Physical causes – trauma, electrocution, lightning strike, suffocation, heat stroke, and gunshot.
Natural or genetic causes – hyperkalemic periodic paralysis, cardiac conductive disturbances, acute myocardial necrosis, cerebral thromboembolism, aortic aneurysm or other vessel rupture, and neoplasia.
Infectious or parasitic causes – acute clostridial diseases, salmonellosis, Tyzzer’s disease, anthrax, equine monocytic ehrlichiosis, foal actinobacillosis, babesiosis, and verminous arteritis.
Metabolic and nutritional causes – hypoglycemia, hypocalcemia, hypomagnesemia, and selenium or vitamin E deficiencies.
CBC/Serum Chemistry/Urinalysis
When possible, collect whole blood, serum, plasma, and urine before death for routine clinicopathologic tests; this helps to delineate pathophysiologic processes, which aids in refining an initial differential list.
Other Laboratory Tests
In addition to samples collected for clinicopathologic testing, other samples for toxicologic analysis include stomach contents, urine, liver, kidney, brain, eyeball, and heart blood. Given the delay in the possible incorporation of a chemical into hair, hair samples are not generally useful for testing.
With any suspicion of an injection site, obtain tissue from around that site.
Collect representative feed and water samples.
Submit plants for identification if necessary (submit fresh whole plants, wrap plant [at least the base of the plant] in moistened paper towels, place in a plastic bag and keep chilled; representative samples of hay can also be collected for identification of contamination).
Because of medicolegal considerations, handle all samples under chain‐of‐custody procedures. These records specifically identify each specimen, document their condition and container in which they are packaged, time and date of both transfer and receipt of samples, and all individuals involved in their handling, transfer, or receipt.
Testing can be targeted or non‐targeted depending on whether there are known exposures or unknown exposures. Mass spectrometry is a powerful technique for broad‐based targeted testing. However, there is no one or two analytical procedures that can rule out all possible chemical exposures.
Pathological Findings
Conduct a complete and thorough postmortem examination.
Consider transporting the animal to a veterinary diagnostic facility as soon as possible. If this is not an option, conduct a thorough field postmortem examination, and record any actual or suspected abnormalities.
If an animal cannot be taken to a veterinary diagnostic facility, consultation with a veterinary pathologist or toxicologist might be warranted to ensure collection and appropriate preservation of useful samples in sufficient quantities.
Carefully examine stomach and GI tract contents for evidence of toxic plant fragments or unexpected grain or forage ingestion.
Collect formalin‐fixed samples from all major organ systems and any gross lesions, and submit these samples to a veterinary pathologist for histopathologic examination.
