Analytical Food Microbiology. Ahmed E. Yousef
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Table of Contents
1 COVER
4 PREFACE
5 PART I: BASICS OF FOOD MICROBIOLOGY LABORATORY CHAPTER 1: LABORATORY SAFETY BACKGROUND PERSONAL SAFETY IN THE LABORATORY MATERIALS IN MICROBIOLOGY LABORATORY PRACTICAL ASPECTS REFERENCE CHAPTER 2: SAMPLING FOR MICROBIOLOGICAL ANALYSIS OF FOOD AND PROCESSING ENVIRONMENT THEORETICAL ASPECTS PRACTICING SAMPLING AND SAMPLE PREPARATION CHAPTER 3: ENUMERATION OF MICROORGANISMS IN FOOD INTRODUCTION PLATE COUNT METHOD MOST PROBABLE NUMBER METHOD SELECTED REFERENCES QUESTIONS CHAPTER 4: PRACTICING BASIC TECHNIQUES INTRODUCTION OBJECTIVES MEDIA PROCEDURE OVERVIEW SELECTED REFERENCES SESSION 1: DILUTION AND PLATING SESSION 2: POPULATION COUNTING AND COLONY STREAKING SESSION 3: MORPHOLOGICAL EXAMINATION
6
PART II: FOOD MICROBIOTA
CHAPTER 5: AEROBIC MESOPHILIC PLATE COUNT
INTRODUCTION
OBJECTIVES
MEDIA
PROCEDURE OVERVIEW
SELECTED REFERENCES
SESSION 1: SAMPLING, HOMOGENIZATION, DILUTION, PLATING, AND INCUBATION
SESSION 2: COLONY COUNTING AND ISOLATION
SESSION 3: EXAMINING COLONY AND CELL MORPHOLOGIES
CHAPTER 6: MESOPHILIC SPOREFORMING BACTERIA
INTRODUCTION
OBJECTIVES
MEDIA AND INCUBATION CONDITIONS
MICROSCOPY METHODS
PROCEDURE OVERVIEW
SELECTED REFERENCES
SESSION 1: SAMPLE PREPARATION, PLATING, AND INCUBATION
MATERIALS AND EQUIPMENT
PROCEDURE
SESSION 2: SPORE ENUMERATION AND EXAMINING SPORULATION
MATERIALS AND EQUIPMENT
PROCEDURE
SESSION 3: MICROSCOPIC EXAMINATION OF SPORES
CHAPTER 7: Pseudomonas SPECIES AND OTHER SPOILAGE PSYCHROTROPHS
INTRODUCTION
OBJECTIVES
MEDIA AND TESTS
PROCEDURE OVERVIEW
SELECTED REFERENCES
SESSION 1: SAMPLING AND PLATING
PROCEDURE
SESSION 2: ENUMERATION OF Pseudomonas SPECIES AND TESTING FOR ENZYMATIC ACTIVITY
PROCEDURE
SESSION 3: DETECTING ENZYMATIC ACTIVITY
SESSION 4: ENUMERATION OF AEROBIC PSYCHROTROPHS
CHAPTER 8: DETECTION AND ENUMERATION OF Enterobacteriaceae IN FOOD
INTRODUCTION
OBJECTIVES
MEDIA AND TESTS
PROCEDURE OVERVIEW
SELECTED REFERENCES
SESSION 1: PREPARATION OF MOST PROBABLE NUMBER TUBES
SESSION 2: PRESUMPTIVE MPN DETERMINATION
SESSION 3: PRESUMPTIVE MPN DETERMINATION AND CONFIRMATION
SESSION 4: CONFIRMATION
CHAPTER 9: EXAMINATION AND ENUMERATION OF FOODBORNE FUNGI
INTRODUCTION
OBJECTIVES