style="font-size:15px;"> Shehriar Husain Department of Dental Materials Science Sindh Institute of Oral Health Sciences Jinnah Sindh Medical University Karachi, Pakistan
Erum Khan CODE‐M Center of Dental Education & Medicine Karachi, Pakistan and Bhitai Dental and Medical College Liaquat University of Medical and Health Sciences, Jamshoro, Pakistan
Syed Ali Khurram Unit of Oral and Maxillofacial Pathology School of Clinical Dentistry University of Sheffield Sheffield, United Kingdom
Zohaib Khurshid Department of Prosthodontics and Dental Implantology College of Dentistry King Faisal University Al Ahsa, Saudi Arabia
Juzer Shabbir Department of Operative Dentistry and Endodontics Liaquat College of Medicine and Dentistry Karachi, Pakistan
Faraz Mohammed Department of Biomedical Dental Sciences College of Dentistry Imam Abdulrahman Bin Faisal University Dammam, Saudi Arabia
Fizza Saher Department of Oral Biology College of Dentistry Ziauddin University Karachi, Pakistan
Rizwan Ullah Department of Oral Biology Sindh Institute of Oral Health Sciences Jinnah Sindh Medical University Karachi, Pakistan
Muhammad Sohail Zafar Department of Restorative Dentistry College of Dentistry Taibah University, Al Madinah Al Munawwarah, Saudi Arabia and Department of Dental Materials Islamic International Dental College Riphah International University Islamabad, Pakistan
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1 Tooth Development
Saqib Ali1, Imran Farooq1, and Syed Ali Khurram2
1 Department of Biomedical Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
2 Unit of Oral and Maxillofacial Pathology, School of Clinical Dentistry, University of Sheffield, Sheffield, United Kingdom
Figure 1.1 H and E stained section showing tooth development.
Tooth development starts on the 37th day of gestation with the formation of primary epithelial bands in the place of future upper and lower jaws. These horse‐shoe‐shaped bands correspond to the future dental arches. These epithelial bands then form two ingrowths called dental lamina (lingually positioned) and vestibular lamina (buccally positioned). These ingrowths extend into the mesenchyme which is surrounded by the neural crest cells. The vestibular lamina proliferates within the mesenchyme and leads to the formation of the vestibule (between the cheek and tooth‐bearing portion of the jaw). The dental lamina gives rise to epithelial outgrowths toward the mesenchyme due to continuous proliferative activity which correspond to the location of forthcoming deciduous teeth. The tooth development is divided into the following stages: bud, cap, and bell (early and late). These stages along with the changes happening in the tooth germ are discussed in detail in the following sections.
1.1 Bud Stage
Figure 1.2 H and E stained section showing the bud stage of tooth development.
Figure 1.3 H and E stained section showing the bud stage of tooth development.
1.1.1 Description
The bud stage is the first stage of tooth development. It represents the first epithelial intrusion into the ectomesenchyme. The cells of the epithelium show minimal changes and the ectomesenchymal cells surround the epithelial bud. Due to the ectomesenchymal condensation (a process in which the epithelial bud propagates into the ectomesenchyme), the density of the cells increases near the epithelial outgrowth. This condensation is owed to the increased mitotic activity carried in the cells of the tooth bud and mesenchymal cells surrounding it. These buds develop at the distal side of the dental lamina and each bud represents a group of cells at dental lamina's end. The epithelial part is separated from the mesenchyme by a basement membrane. The ectomesenchyme surrounding the tooth bud is called the dental follicle or sac whereas the area directly subjacent to the condensation is called the dental papilla. The dental follicle is ultimately responsible for the formation of cementum, periodontal ligament (PDL), and alveolar bone. The dental papilla is responsible for the formation of dental pulp and dentin.
1.1.2 Key Identifying Features
The enamel organ at this stage appears roughly ovoid to spherical with poor histodifferentiation and morphodifferentiation. A typical tooth bud consists of centrally located polygonal (multiple‐shaped) cells and peripherally arranged columnar cells.
1.1.3 Clinical Significance
The successful development of the tooth depends on the interaction of epithelial and mesenchymal components. If these parts grow individually, neither will differentiate further [1]. This epithelial–mesenchymal interaction starts in the bud stage; therefore, any problem affecting the bud stage could seriously affect the development of teeth.
1.2 Cap Stage
Figure 1.4 H and E stained section showing the cap stage of tooth development.
