The Peripheral T-Cell Lymphomas. Группа авторов
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Specific Microenvironment Components Present in Other Primary Cutaneous T‐cell Lymphoma Entities
A microenvironment component is present in all PTCL entities. Here, we review the data relative to distinct cellular components.
Macrophages
A high content in histiocytes is present in some PTCL entities. For example in the lymphohistiocytic variant of ALCL ALK‐positive, which occurs exclusively in children and young adults, the gene expression signature is largely contributed by the histiocytic component and, in comparison to the classical disease the patients have a more disseminated disease with tendency to a leukemic picture and a worse prognosis with a high risk of failure [77]. Conversely, the lymphoepithelioid variant of PTCL, NOS tends to be associated with an overall better prognosis than other PTCL‐NOS [78, 79]. In PTCL, NOS rich in histiocytes, the molecular signature related to inflammatory response (chemokines, cathepsins, major histocompatibility complex (MHC) molecules, genes involved in the interferon response pathway) and to the monocyte–macrophage background appears to be inversely related to a proliferation signature and associated with an adverse prognosis [80, 81].
Angiogenesis
Among non‐Hodgkin lymphomas, PTCLs demonstrate the highest microvessel density [82]. Increased microvessel density has also been shown in cutaneous biopsies involved by mycosis fungoides in comparison with inflammatory skin conditions [83]. VEGF overexpression in PTCL, NOS has been associated with a poor outcome [84]. VEGF may result in local neovascular transformation (angiogenesis) and recruitment of circulating progenitors derived from the bone marrow (vasculogenesis) [85]. However, in contrast to AITL, it is unclear which cell type(s) release VEGF and whether VEGF receptors are present and functional on the lymphoma cells in PTCL, NOS. In models of NPM‐ALK‐driven oncogenesis, STAT3 induces VEGF expression by the lymphoma cells. MicroRNA‐135b is another mediator of NPM‐ALK‐mediated angiogenesis [86]. Elevated serum VEGF levels in patients with non‐Hodgkin lymphoma have been reported to be associated with a poor outcome [87]. Attempts to improve the outcome of patients with PTCL by adding bevacizumab have, however, proven ineffective [88].
Figure 2.2 Neoplastic T follicular helper cells and their microenvironment in angioimmunoblastic T‐cell lymphoma. B, B cell; EBV, Epstein–Barr virus; FDC, follicular dendritic cell; IL, interleukin; LT‐β, lymphotoxin‐beta; MAC, macrophage; PC, plasma cell; TFH, T follicular helper cells; TGF, transforming growth factor; Th1, T lymphocyte 1; Treg, T regulatory cells (gray arrows: epigenetic mutations, which may occur early and may be detected in neoplastic cells and other reactive cells including B cells; asterisk: additional mutations or somatic hypermutations; red arrows: T‐cell‐specific mutations like RHOA G17V or others.
Source: Adapted from de Leval and Gaulard [63].
Eosinophils
Blood and tissue eosinophilia may be seen in various PTCLs as a result of non‐clonal expansion of normal eosinophils mediated by eosinophilopoietic growth factors, such as IL3, granulocyte‐macrophage colony stimulating factor and IL5 normally produced by activated T cells (for review see Roufosse F et al. [89]). Other factors promoting eosinophil chemotaxis include RANTES (regulated on activation normal T cell expressed and secreted)/CCL5 and eotaxins 1–3 (CCL11, CCL24, CCL26). RANTES also exerts chemoattractant activity for T lymphocytes, monocytes, and basophils, and is produced by a variety of cell types, including T cells, fibroblasts, and epithelial cells. Eotaxins signal through CCR3 receptor, which is expressed at high levels on eosinophils and Th2 cells. Cellular sources of eotaxin include fibroblasts, endothelial cells, eosinophils, and lymphocytes.
PTCLs most commonly associated with eosinophilia include primary CTCL, AITL, and ATLL. Approximately 15–20% of patients with mycosis fungoides and up to 75% of those with Sézary syndrome develop blood eosinophilia and show numerous eosinophils within cutaneous lymphoma infiltrates. Eosinophilia in mycosis fungoides and Sézary syndrome, is related to the Th2 nature of the lymphoma cells, which produce IL4, IL5, and IL13. The recruitment of eosinophils to the skin is mediated by chemoattractants produced by the lymphoma cells but also possibly by other cell types. Indeed, IL4‐producing lymphoma cells induce increased expression of eotaxin‐3/CCL26 by keratinocytes, endothelial cells, and fibroblasts [89]. In cutaneous ALCL as well lymphomatoid papulosis (type A), a reactive inflammatory infiltrate may be found within the tumor, and eosinophils may be detected in a substantial proportion of patients occasionally representing the predominant cell type. CD30+ tumor cells isolated from skin biopsies with cutaneous ALCL have been shown to coexpress CCR3 and IL4, and eotaxin in conjunction with surrounding cells, indicating that they contribute to eosinophilic infiltrates [90].
The functional role of eosinophils within the tumor microenvironment remains poorly characterized. Eosinophils also produce IL5 and since they also express the cognate receptor, autocrine activation is possible. Clinical and experimental investigations have shown that eosinophils can function as antigen‐presenting cells and can promote the proliferation of effector T cells. In addition, eosinophils are able to produce an array of cytokines (IL2, IL4, IL6, IL10, and IL12) capable of promoting T‐cell proliferation, activation, and influencing Th1–Th2 polarization, thereby regulating tumor cell growth and expansion [91].
Underlying Factors Favoring the Tumor Transformation
Mechanisms driving cancer initiation and genomic instability are unclear in PTCL. Indeed, in contrast to other cancers where critical susceptibilities have been identified, such as tobacco or human papillomavirus in lung or cervix carcinomas, or the importance of AID in B‐cell malignancies, few factors are known to predispose to PTCL.
Viruses
Two viruses with oncogenic properties, HTLV1 and EBV play a causal role in the development of NK‐ or T‐cell lymphoproliferations.
Human T‐cell Leukemia Virus Type 1
HTLV1, also known as human T‐lymphotropic virus type 1, was the first exogenous human deltaretrovirus discovered [92]. The 9‐kb genome of HTLV1 encodes the gag, pol, and env structural proteins, plus accessory and regulatory proteins at the 3′ end of the genome (tax, rex, p12, p21, p30, p13, and HBZ) that play an important role in the regulation of viral replication, persistence, and leukemogenesis [93].
HTLV1 infection causes ATLL, after a long latency of about 50 years after mother‐to‐child transmission. ATLL is a disease exemplifying an infectious agent‐driven lymphoma and multistep lymphomagenesis. Only a small fraction of infected individuals will ever develop ATLL, while the