under pressure, and a substantial hematoma may develop quickly. The signs of this are pain, discoloration, or oozing at the venipuncture site. If this occurs, the blood flow is discontinued immediately, pressure is applied, and the hematoma is managed as described for whole blood donation.
Mechanical hemolysis
Because blood is pumped through tubing and centrifuges of various configurations, hemolysis is a theoretical complication due to constricted tubing or the geometry of the flow pathways. When this rare event occurs in practice, generally a defective collection kit is suspected [91, 92].
Platelet depletion or damage
Plateletpheresis does not damage the donor’s remaining platelets, and the donor’s platelet function is normal after donation [93]. Removal of platelets equivalent to several units of the donor’s blood does not result in thrombocytopenia. In a rather dramatic example, a female donor underwent 101 donations during a 33‐month period, with donation frequencies ranging from once to three times weekly [94]. Her platelet count remained in the range of 135,000–430,000 during this time. In donors who undergo repeated plateletpheresis, the platelet count decreases somewhat more, but then stabilizes [78, 95]. Thus, it appears that platelets can be donated safely approximately every 2–4 days if needed.
Lymphocyte depletion
Instruments and procedures have been improved to minimize the leukocyte content in the product, and thus also avoid potential complications of lymphocyte depletion in the donor. As a result, most plateletpheresis procedures today remove about 1 × 106 to 5 × 107 leukocytes. Loss of this number of leukocytes is very unlikely to lead to leukocyte depletion or any clinical effects on the donor’s immune function and has the advantage of producing a leukodepleted product. This is true even after serial collections completed in a relatively short period [96, 97]. However, in studies using the previously employed methods, significant lymphocyte depletion was observed together with decrease in total lymphocyte count and B and T cells, lower T4/T8 ratio, and reduced mitogen response [98–100]. Separate studies indicated that at least 109 lymphocytes must be removed daily to observe a significant decline in lymphocyte count and at least 1011 lymphocytes total must be removed over a short time and/or the individual’s lymphocyte count must be less than 500/μL for clinical immunosuppression to occur [97, 101]. The most recent studies show that although there is variability in lymphocyte loss in serial donors with the two most common collection instruments, the loss does not appear to lead to any clinical predisposition to infection [102–104]. Although one study suggests a possible increased risk of immunosuppression related infections [105].
Complications unique to granulocyte donation
The complications related to leukapheresis itself are not different from those in plateletpheresis, but donors receive HES, corticosteroids, and possibly G‐CSF that can cause additional problems. Although there are only a few studies of side effects in frequent granulocyte donors [106], experience with stem cell donors has also provided information about side effects [107]. Short‐term effects of HES include rashes and fluid shifts, while the effects of corticosteroids and G‐CSF are also well known and are discussed in Chapter 6. Although there have been concerns about long‐term effects, such as cataracts [108, 109] or malignancy [110], in repeat donors, these have not been confirmed in studies.
Complications unique to plasmapheresis
One unexpected risk from plasmapheresis, especially when done frequently for therapeutic purposes, is the development of anemia, which is probably due to the blood samples used for laboratory testing [111].
Complications unique to mononuclear cell apheresis for collection of peripheral blood stem cells
Because of the low level of circulating PBSCs in normal donors, donors receive the hematopoietic growth factor G‐CSF to mobilize PBSCs and increase the yield. G‐CSF is also often used, sometimes in combination with glucocorticoids, to increase yields for granulocyte collections. G‐CSF, itself, is associated with a high frequency of side effects and is responsible for most of the complications of the donation of mononuclear cells as a source of PBSCs [107].
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