Bovine Reproduction. Группа авторов
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In addition, Sertoli cells secrete two closely related protein complexes of the transforming growth factor cytokine superfamily (activin and inhibin) that have opposite regulatory effects on the synthesis and regulation of pituitary FSH secretion. Activin upregulates while inhibin downregulates FSH synthesis and secretion [123, 124]. Both of these hormones are secreted after puberty and play an important role in mammalian reproduction, which is coordinated by assorted neural, neuroendocrine, endocrine, and paracrine cell–cell communication pathways [125]. Activin is also thought to facilitate androgen synthesis in the testis by enhancing LH, thereby promoting spermatogenesis. In male mammals, inhibin production is thought to be promoted by testicular androgens and may function locally in the testis to help regulate spermatogenesis. Much less is known about inhibin in relation to its mechanism of action, but it is thought that it may compete with activin for the binding of the activin receptor and/or binding to the inhibin‐specific receptor [124]. However, Phillips [126] in reviewing the literature notes that inhibin not only acts as a feedback regulator of FSH in the male, but also may be an important paracrine and autocrine regulator of testis function. It has been shown from molecular studies that both forms of inhibin (A and B) are produced by the bovine testis and that the proportion of the mature forms of inhibin A and inhibin B increases as bulls age, but that total inhibin production by Sertoli cells decreases [127]. In a later study, Kaneko et al. [128] reported that inhibin A and inhibin B increased in the testis of bulls during postnatal development, and that immunoreactive inhibin A could be detected in the plasma of these bulls. Others have attempted to use blood concentrations of inhibin and gene expression along with other endocrine and genetic markers as a predictor of fertility in Brahman bulls [129]. Unfortunately, there appears to be little information in the current literature on the role activin plays in the bovine male. Its role as a paracrine regulator in the testis is presumed but has not been conclusively demonstrated in domestic species, although in vitro studies in porcine Leydig cells would suggest a role in androgen synthesis [126]. However, it is thought to be an important intrafollicular factor in the regulation of follicle selection in the bovine female [130, 131]. A role in inflammatory processes for activin has been postulated [126].
Additional factors secreted by the Sertoli cell include ABP, estradiol (by conversion of testosterone via the aromatase enzyme complex), glial cell‐derived neurotrophic factor (GDNF), transferrin [132], and the more recently identified ERM transcription factor. Testicular transferrin and ABP are two glycoproteins produced and secreted into the lumen of the tubule by Sertoli cells, and which have important biological effects on differentiation and maturity of sperm [133, 134]. ABP is a glycoprotein that binds specifically to testosterone, DHT, and 17β‐estradiol. By binding to ABP, the steroid hormones testosterone and DHT are less lipophilic and become more concentrated within the lumen of the seminiferous tubules, thereby helping to facilitate spermatogenesis. ABP synthesis is regulated by FSH activity on the Sertoli cells, which may be enhanced by insulin and testosterone. ABP can especially combine with testosterone to maintain a high concentration of androgen in the seminiferous tubule, which facilitates the development and maturation of spermatogenic cells. Androgen is transferred to the target cells and via the nuclear transfer mechanism into the nucleus to combine with androgen receptors, which regulate translation of the target genes. The characteristic of ABP combining with androgen shows that ABP participates in sexual differentiation and maturation of germ cells [132].
Sertoli cell gene products include growth factors, metabolic enzymes, transport proteins (e.g. transferrin, ABP), inhibin, proteases, antiproteases, energy metabolites, and structural components [135]. Transferrin is a major secretory product of differentiated Sertoli cells and is postulated to transport iron sequestered by the blood–testis barrier to the developing germ cells. Fe3+ in blood is transferred to Sertoli cells, combined with transferrin synthesized by Sertoli cells, and further transferred to spermatogenic cells at the development stage to promote germ cell growth and maturation. It is well known that the synthesis and secretion of transferrin and ABP are regulated by FSH and androgen. The gonadotropin FSH is an important endocrine hormone required for the regulation of Sertoli cell function [136]. FSH has been shown to regulate the expression of most Sertoli cell genes, including the FSH receptor, ABP, transferrin, plasminogen activator, and aromatase [137–140].
GDNF is a small protein normally associated with the promotion of neuron survival [141]. Its role in testicular function is not fully understood, but there is some evidence to suggest that it may be involved in promoting undifferentiating spermatogonia, which ensures stem cell self‐renewal during the perinatal period [142]. Moreover, Johnston et al. [143] demonstrated in the adult rat that cyclic changes in GDNF expression by Sertoli cells are responsible for the stage‐specific replication and differentiation of stem spermatogonia, the foundational cells of spermatogenesis. Recent reports in the literature have demonstrated that GDNF, rather than induce proliferation of spermatogonia stem cells, enhances self‐renewal and increases survival rate of bovine spermatogonia in culture [144]. An interesting observation reported by Harikae et al. [145] is a rare case of a freemartin calf exhibiting the transdifferentiation of ovarian somatic cells into testicular somatic cells including Sertoli cells, Leydig cells, and peritubular myoid cells, and that the Sertoli cells stained positive for GDNF protein. They speculate that these observations suggest that mammalian XX ovaries may have a high potential for sexual plasticity. For a more comprehensive review of the function and regulation of Sertoli cells, the reader is referred to the excellent review by Russell and Griswold [146].
Overview of Spermatogenesis and Maturation of Spermatozoa
The first studies on the process of spermatogenesis and the spermatogonia of the domestic bull were reported in the latter part of the nineteenth century [147], but it was not until 1931 that the chromosome number of spermatogonia and spermatocytes was determined and that the diploid number was 60 [148]. Subsequent studies in the era after the Second World War described the germ cells and their cytoplasmic structures in more detail [147]. It is now well established that spermatogenesis is a complex cellular process whereby spermatozoa, the male haploid germ cell or gamete, are formed from the diploid spermatogonia stem cells through a series of cellular transformations. These complex transformations occur in the seminiferous tubules of the mammalian testes and may proceed over an extended period of time, which is species dependent. Spermatogenesis has been described morphologically in distinct and recognizable cellular “stages” or “phases” that progress through highly organized and precisely timed cycles [35]. During fetal development primordial germ cells migrate to the embryonic testes where they undergo mitotic division to form gonocytes. Just prior to puberty, gonocytes differentiate into the primary pool of A0 spermatogonia, the stem cells from which all subsequent classes of spermatogonia arise. Spermatogenesis proceeds through three distinct stages within the seminiferous tubules of the testis. The first stage is spermatocytogenesis, a proliferative phase where spermatogonia undergo a series of mitotic divisions to form primary spermatocytes. The second stage is meiosis where the primary spermatocytes undergo reduction division of the chromosomal number, from primary spermatocytes (4n) to secondary spermatocytes which are diploid (2n) to the final division that produces round