b) the progression of furrowing was disturbed, resulting in total blockage, if the furrow had not already progressed over more than the half of the respective cells.
Experiments with the F-Actin-affecting inhibitor cytochalasin B (0,05 - 2,5 µg/ml) showed that a) cytokinesis was not blocked, b) adhesion of the blastomeres to each other was often dissolved and c) toxic effects (cell blebbing and malformation) could occur.
Staining with rhodaminyl phalloidin to show F-Actin and subsequent fluorescence microscopical examination could not produce any evidence for a "contractile ring" or "arch" located beneath the furrow.
Keywords
Hydractinia echinata, anti-tubulin, blastomeres, cell division, cellular movements, cleavage furrow formation, cytochalasin B, cytokinesis, cytoskeleton, DAPI (4',6-Diamidino-2-Phenylindole), developmental biology, Diplom (graduation thesis at German unversity), early development, F-actin, fluorescence microscopy, furrowing, Hydrozoa, inhibitor, marine, marine biology, mechanism of cell division, microtubuli, mitosis, nocodazole, normal development (normogenesis), polyps, rhodaminyl phalloidin, zoology
Inhalt
Beschreibung und Zusammenfassung
2.1 Hälterung der Versuchstiere
2.3 Behandlung und Beobachtung der Keime
2.4 Aufbringen von Farbmarkern
2.6.2 Zur Rhodaminylphalloidin-Färbung
2.6.3 Zur indirekten Immunfluoreszenz mit dem Anti-Tubulin-Antikörper WA3
2.6.4 Fixierungs- und Färbeprozedur: Methode 1
2.6.5 Fixierungs- und Färbeprozedur: Methode 2
2.7 Versuche mit Hemmstoffen des Cytoskeletts
2.8 Lichtmikroskopisches Material und Technik
2.10 Rasterelektronenmikroskopie (REM)
2.11 Transmissionselektronenmikroskopie (TEM)
2.12 Fotografische Dokumentation
3.1 Vorbemerkung: Fluoreszenzmikroskopie bei Hydractinia-Keimen
3.3.1 Karyogamie und erste Prophase
