Practical Guide to Diagnostic Parasitology. Lynne Shore Garcia

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       PRACTICAL GUIDE TO Diagnostic Parasitology

       SECOND EDITION

       SECOND EDITION

       PRACTICAL GUIDE TO Diagnostic Parasitology

       Lynne S. Garcia M.S., MT(ASCP), CLS(NCA), F(AAM) LSG & Associates, Santa Monica, California

      Address editorial correspondence to ASM Press, 1752 N St. NW, Washington, DC 20036-2904, USA

      Send orders to ASM Press, P.O. Box 605, Herndon, VA 20172, USA

      Phone: 800-546-2416; 703-661-1593

      Fax: 703-661-1501

      E-mail: [email protected] Online: estore.asm.org

Copyright © 2009 ASM PressAmerican Society for Microbiology1752 N St. NWWashington, DC 20036-2904

      Library of Congress Cataloging-in-Publication Data

      Garcia, Lynne Shore.

      Practical guide to diagnostic parasitology / Lynne S. Garcia. — 2nd ed.

       p. ; cm.

      Includes bibliographical references and index.

      ISBN 978-1-55581-454-0 (softcover : alk. paper) 1. Diagnostic

      parasitology. I. Title.

      [DNLM: 1. Parasitic Diseases—diagnosis. 2. Laboratory Techniques and

      Procedures. WC 695 G216p 2009]

      RC255.G37 2009

      616.9′6075—dc22 2008047918

      Current printing (last digit)

      10 9 8 7 6 5 4 3 2 1

      All Rights Reserved

      Printed in the United States of America

      Cover figure: Bloodstream forms of the African trypanosome, Trypanosoma brucei, stained for a surface membrane protein (in green) and counterstained with the DNA intercalating dye DAPI, thus revealing the parasite’s central nucleus and posterior kinetoplast. (Courtesy of Keith Matthews and Sam Dean, University of Edinburgh.)

       I dedicate the second edition of this practical guide to Robyn Shimizu. Robyn and I have worked together for many years, performing bench work, training students, presenting workshops and seminars, handling consults, performing studies, and preparing manuscripts for publication. A very special thanks to Robyn for her collaboration and support—it’s been quite an adventure, and we’re looking forward to continued collaboration.

       Contents

       Preface

       Acknowledgments

       SECTION 1 Philosophy and Approach to Diagnostic Parasitology

       Why Perform This Type of Testing?

       Travel

       Population Movements

       Control Issues

       Global Warming

       Epidemiologic Considerations

       Compromised Patients

       Approach to Therapy

       Who Should Perform Diagnostic Parasitology Testing?

       Laboratory Personnel

       Nonlaboratory Personnel

       Where Should Diagnostic Parasitology Testing Be Performed?

       Inpatient Setting

       Outpatient or Referral Setting

       Decentralized Testing

       Physician Office Laboratories

       Over-the-Counter (Home Care) Testing

       Field Sites

       What Factors Should Precipitate Testing?

       Travel and Residence History

       Immune Status of the Patient

       Clinical Symptoms

       Documented Previous Infection

      

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