2.16a). The point transect approach is similar, however only the species at a predetermined distance along the line is measured, for example, every 20 cm (Fig. 2.16b).
Fig. 2.16 (a) Line transect record card. (b) Point transect record card.
Source: Sara Vero.
Event Sampling
Event sampling is perhaps most common in hydrology and related fields. This approach involves taking samples during or subsequent to specific target conditions. This frequently involves measurement of surface water quality in response to storms, which generate overland flow and runoff of potential contaminants such as sediment or pesticides to surface water, although subsurface leaching and preferential flow may also be triggered under such conditions. Event sampling will involve fairly typical techniques; the key difference is in preparedness and timing. As events can be both sudden and relatively brief, if you are not completely prepared in advance you may miss them. There are some steps which can help:
You can set email or text alerts either linked to weather stations or dataloggers at your site or linked to meteorological forecasts from reliable sources.
Have a grab‐bag of tools and equipment so that you can deploy to the field at short notice.
Know how long it takes for you to safely get to your site, and how long you need to conduct your sampling. Your alert must be far enough in advance to allow you to get there. If it takes 36 h to reach your site, for example, a 24‐h weather warning will be of no use as it will be impossible for you to reach the site in time to capture the event.
Keep your vehicle fueled.
If your site is distant or if you have a good general estimate of when a future event might occur (e.g., which month typically receives most storms), consider deploying to the field or to a nearby location in advance. It may be more effective to stay at a hotel near the location and limit the risk of missing the event.
Be safe! Event sampling may pose elevated risks for several reasons that should be considered when preparing your hazard and risk assessment:Weather might be cold, wet, and windy, making driving conditions poor.Stream or river discharge will be high, elevating the risk of being washed away, drowning, and slips or trips.You might feel rushed and take more risks, be less thorough, and feel more stressed or distracted.
Grab Versus Composite Sampling
Grab sampling is when a sample is taken at a specific location and single point in time that provides a “snapshot” of that specific moment. Frequently, grab samples are subject to environmental conditions prior to sampling and may be strongly influenced by incidents occurring recently. However, grab sampling has a valuable role in environmental research. It can be used in scoping studies to indicate the suitability of a site for future research or to evaluate conditions subject to an event. Grab samples may be incorporated into case studies or site characterization, which can provide useful additional background data to support analysis. Heterogeneity is the enemy of grab sampling! Where sites are highly heterogeneous, an unstructured approach to characterization will rarely yield an accurate understanding. To make matters worse, if there is a significant element of sorting, you may be predisposed to sampling a specific component. Therefore, the more heterogeneous or dynamic a site, material or process is, the less suitable grab sampling is as a method of characterization. You should also consider the type of heterogeneity; compositional or distributional.
Compositional heterogeneity is when a population is made up of several different components or elements, which can be in equal or differing proportions, but which is mixed. Consider a meadow in which grass and flower species are randomly distributed with no particular structure. In this instance, a single quadrat of 1 m2 cut at any location in that meadow would be likely to harvest a variety of different species, however, because of randomness could not be guaranteed to reflect the overall composition of that sward.
Distributional heterogeneity is when a population of different components is structured or distributed. Imagine if the same species from the meadow were separated out and each species planted in rows of only their kind. In that instance, a quadrat cut at any location would only harvest a single species, and definitely would not reflect the overall sward composition. Distributional heterogeneity also applies to variations which occur over time, for example, diurnal fluctuations.
Both types of heterogeneity are poorly captured by grab sampling. Another example is loads in a watercourse. If the concentrations are relatively stable (i.e., do not fluctuate depending on flow or time), then grab sampling should be an acceptable indicator of water quality. However, if there is heterogeneity, such as what may arise from diurnal nutrient discharges, or if there is a dilution effect during high‐flow periods, then a grab sample could not reliably indicate quality or loads. However, a modified approach to grab sampling can be used to provide a synoptic snapshot; in other words, a more thorough picture of a scenario at a specific point in time. An example of this is taking a large number of water samples across a watercourse at the same time or within a very short period (hours) during which flow is stable. Each sample remains discrete and each are non‐replicated, but viewed altogether, can be used to investigate patterns across a watershed.
Composite sampling consists of multiple samples taken over a period of time or across an area. In other words, composite sampling is essentially incorporation of multiple grab samples and treating this aggregate as a single unit. It is important that representative amounts of each grab sample are present in the composite, otherwise it will be biased. A composite approach may require greater effort but has the advantage of being more representative of the area or process. Examples of composite sampling are:
Taking multiple samples of surface soil, mixing thoroughly and analyzing for extractable phosphorus to indicate the fertilizer requirement of that field.
Taking hourly samples of streamwater using an autosampler (Fig. 2.17) to assess total load of nitrate; due to diurnal fluctuations, large grab samples will not be reflective (see Facchi et al., 2007).Fig. 2.17 The autosampler shown here uses a pump to extract water samples from the stream below at scheduled or flow‐weighted intervals. This allows grab or composite samples to be taken without the presence of the researcher.Source: Sara Vero.
Replicated sampling also involves taking several discrete measurements or samples, but unlike composite sampling, each replicate is treated as an individual and is not mixed with the others. The statistical difference between the replicates should be assessed.
Sampling for Laboratory Studies
Fieldwork doesn't necessarily mean that the experiment itself is conducted outdoors. You may need to collect materials for use in controlled, laboratory tests. For example, studies of microbial respiration using incubation chambers need substrate such as soil, sediment, manure, etc. Unlike agar,
