href="#u52a37d4c-4e03-5ee8-9c6c-d33c176f59db">Chapter 23), and continues with a chapter dealing with viral genomics and bioinformatics (Chapter 24). We intend that these three chapters will give our students insight into the current threads of molecular and virological thinking. Part V concludes with our chapter on “Viruses and the Future” (Chapter 25), containing updated material on emerging viruses, including influenza, as well as viruses and nanotechnology.
A major change in this edition is the use of full‐color illustrations. We welcome this effort from our publisher, Blackwell Science, and hope that you find this adds value and utility to our presentation.
In conjunction with the expanded coverage, the Glossary has been revised. In addition, all of the references, both text and web‐based, have been reviewed and made current as of this writing.
Most of these changes were either finished or discussed in detail before Ed's untimely passing. As a result, we are proud to say that Basic Virology, Third Edition, bears the welcome imprint of the scientist/teacher who inspired the first one. We hope you agree and enjoy the fruits of this effort.
Marty Hewlett, Taos, NM, USA
Dave Bloom, Gainesville, FL, USA
David Camerini, Irvine, CA, USA
Preface to the Fourth Edition
It seems like only yesterday that Ed and Marty spoke on the phone and said, in effect, “Let's do this thing,” giving birth to Basic Virology. And here we are, completing the revisions for the fourth edition of what we hope will remain a useful and relevant textbook for the teaching of introductory virology to undergraduates.
As it has from its very beginnings, the field of virology is changing at an astounding pace, with newly recognized diseases and their viral causes being reported, accompanied by ever more sophisticated techniques for studying these entities that exist at the fringes of the living world.
In this latest edition we have attempted to capture some of this dynamism, while retaining the organization and pedagogical approach of the original. To that end we have added new and expanded discussions of such agents as Ebola virus, Zika virus, and H1N1 and H7N9 influenza virus, as well as the SARS‐CoV‐2/COVID‐19 pandemic, with information that is current as of this writing. We have modified our presentation of techniques, removing some that are outdated (CoT curves, as an example), retaining the classics that have defined the field (pulse and pulse‐chase labeling), and introducing the newest approaches that are opening new areas of investigation (CRISPR‐Cas).
The organization of the book has been retained from the third edition, with 25 chapters divided into five parts, including the Case Studies, updated as necessary. We have tried to avoid textbook size creep by making judicious editorial choices. Figures have been changed as needed to reflect new information, with the addition of new graphics where necessary to complete new or expanded coverage.
We hope that you find this version of our work both useful and relevant in your teaching of our favorite topic . . . virology!
David Camerini, Martinez “Marty” Hewlett, and Dave Bloom: Michael's Kitchen and Bakery, Taos, New Mexico, March 2017.
Acknowledgments
Even the most basic text cannot be solely the work of its author or authors; this is especially true for this one. We are extremely grateful to a large number of colleagues, students, and friends. They provided critical reading, essential information, experimental data, and figures, as well as other important help for all four of the editions of Basic Virology. This group includes the following scholars from other research centers: Wah Chiu, Stanford University; J. Brown, University of Virginia; J. B. Flannegan and R. Condit, University of Florida; J. Conway, National Institutes of Health; K. Fish and J. Nelson, Oregon Health Sciences University; D. W. Gibson, Johns Hopkins University; P. Ghazal, University of Edinburgh; H. Granzow, Friedrich‐Loeffler‐Institut, Insel Riems; C. Grose, University of Iowa; J. Hill, Louisiana State University Eye Center–New Orleans; S. Karst, University of Florida; J. Langland, Arizona State University; D. Leib, Dartmouth College; F. Murphy, University of California, Davis; S. Rabkin, Harvard University; S. Rice, University of Alberta–Edmonton; S. Silverstein, Columbia University; B. Sugden, University of Wisconsin; Gail Wertz, University of Alabama–Birmingham; and J. G. Stevens, University of California, Los Angeles. Colleagues at the University of California, Irvine who provided aid include R. Davis, S. Larson, A. McPherson, T. Osborne, R. Sandri‐Goldin, D. Senear, B. Semler, S. Stewart, W. E. Robinson, I. Ruf, and L. Villarreal. Both current and former workers in Edward Wagner’s laboratory did many experiments that aided in a number of illustrations; these people include J. S. Aguilar, K. Anderson, R. Costa, G. B. Devi‐Rao, R. Frink, S. Goodart, J. Guzowski, L. E. Holland, P. Lieu, N. Pande, M. Petroski, M. Rice, J. Singh, J. Stringer, and Y.‐F. Zhang. Colleagues of David Camerini that did experiments and helped make figures used in the fourth edition are Joseph J. Campo, Shailesh K. Choudhary, Arlo Randall, and Robert M. Scoggins.
We were aided in the writing of the second edition by comments from Robert Nevins (Milsap College), Sofie Foley (Napier University), David Glick (King’s College), and David Fulford (Edinboro University of Pennsylvania).
We want to remember the many people who contributed to the physical process of putting the first edition of this book together. R. Spaete of the Aviron Corp carefully read every page of the manuscript and suggested many important minor and a couple of major changes. This was done purely in the spirit of friendship and collegiality. K. Christensen used her considerable expertise and incredible skill in working with us to generate the art. Not only did she do the drawings, but also she researched many of them to help provide missing details. Two undergraduates were invaluable to us. A. Azarian at University of California, Irvine made many useful suggestions on reading the manuscript from a student’s perspective, and D. Natan, an MIT student who spent a summer in Edward Wagner’s laboratory, did most of the Internet site searching, which was a great relief and time saver. Finally, J. Wagner carried out the very difficult task of copyediting the manuscript.
From the beginning, a number of people at Blackwell Science represented by Publisher N. Hill‐Whilton demonstrated a commitment to a quality product. We especially thank Nathan Brown, Cee Brandson, and Rosie Hayden, who made great efforts to maintain effective communications and to expedite many of the very tedious aspects of this project. Blackwell Science directly contacted a number of virologists who also read and suggested useful modifications to this manuscript: Michael R. Roner, University of Texas, Arlington; Lloyd Turtinen, University of Wisconsin, Eau Claire; and Paul Wanda, Southern Illinois University.
All of these colleagues and friends represent the background of assistance we have received, leading to the preparation of this fourth edition. We would especially like to acknowledge Dr. Luis Villarreal and the Center for Virus Research at the University of California, Irvine for supporting our efforts in bringing this book to a timely completion.
PART I
Virology
