Liquid‐phase matrix enzyme immunometric system using avidin‐biotin interaction to capture allergen‐specific IgE combined with solid‐phase ELISA methodology to quantify IgE
Serum
Quantitative assay to measure allergen‐specific IgE
Hair, skin scrapings (from active border of lesion)
Dermatophyte (Microsporum spp. or Trichophyton spp.) Positive results indicate infection when clinical lesions are present, but indicate nonclinical carriage state without clinical lesions. Negative results indicate no or few Microsporum spp. or Trichophyton sp. organisms in sample. Because positive PCR results can be due to active infection, fomite carriage or nonviable fungal organisms from a successfully treated infection, PCR for dermatophyte may be most useful for initial diagnosis, while fungal culture may be a better indicator of mycologic cure. Note: False negatives have been seen with this test, so culture is still recommended
Measures presence of anti‐Sarcoptes antibodies in serum. Positive results indicate possible Sarcoptes infection, or false positive due to house dust mite hypersensitivity (these antigens cross react). Dogs may remain positive for up to 6 months after successful treatment. Negative results indicate lack of exposure, early infection, or immunosuppressive medication interactions. Sensitivity is 84.2%, and specificity is 89.5%
Cornell University College of Veterinary Medicine, Animal Health Diagnostic Center PCR
Indicates the presence of DNA from Ehrlichia canis, Neorickkettsia risticii, Anaplasma phagocytophilum, Bartonella spp., hemotropic Mycoplasma spp. Many additional individual PCR tests available on website
Indicates the presence of DNA from Anaplasma spp., Babesia spp., Bartonella spp., canine hemotropic Mycoplasma, Ehrlichia spp., Hepatozoon spp., Leishmania spp., Neorickettsia risticii, and Rickettsia rickettsii. Quantifies DNA of Leishmania spp.
Measures antibodies to Borrelia burgdorferi, Anaplasma sp., and Ehrilichia sp., and measures Dirofilaria immitis antigen Measures presence of DNA from several tick‐borne organisms, and measures Borrelia burgdorferi antibodies. Also offers B. burgdorferi PCR and Western Blot
Provided by many research institutions and universities. Polymerase chain reaction (PCR) tests are available for common dermatologically relevant bacteria, including Staphylococcus spp., Pseudomonas sp., Streptococcus sp., E. coli, Bordetella sp., Mycobacteria spp., Bartonella sp., etc. Refer
www.idexx.com
