Cas9 nucleases. Nat. Biotechnol. 31: 827–832.33 Jaitin, D. A., Weiner, A., Yofe, I., Lara‐Astiaso, D., Keren‐Shaul, H., David, E., Salame, T. M., Tanay, A., Van Oudenaarden, A. & Amit, I. 2016. Dissecting immune circuits by linking CRISPR‐pooled screens with single‐cell RNA‐Seq. Cell, 167, 1883–1896 e15.
34 Joberty, G., Falth‐Savitski, M., Paulmann, M. et al. (2020). A tandem guide RNA‐based strategy for efficient CRISPR gene editing of cell populations with low heterogeneity of edited alleles. The CRISPR J. https://doi.org/10.1089/crispr.2019.0064.
35 Jost, M., Chen, Y., Gilbert, L.A. et al. (2017). Combined CRISPRi/a‐based chemical genetic screens reveal that Rigosertib is a microtubule‐destabilizing agent. Mol. Cell 68: 210–223. e6.
36 Kamens, J. (2015). The addgene repository: an international nonprofit plasmid and data resource. Nucleic Acids Res. 43: D1152–D1157.
37 Katigbak, A., Cencic, R., Robert, F. et al. (2016). A CRISPR/Cas9 functional screen identifies rare tumor suppressors. Sci. Rep. 6: 38968.
38 Kim, S., Kim, D., Cho, S.W. et al. (2014). Highly efficient RNA‐guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins. Genome Res. 24: 1012–1019.
39 Kim, D., Luk, K., Wolfe, S.A., and Kim, J.S. (2019). Evaluating and enhancing target specificity of gene‐editing nucleases and deaminases. Annu. Rev. Biochem. 88: 191–220.
40 Kimberland, M.L., Hou, W., Alfonso‐Pecchio, A. et al. (2018). Strategies for controlling CRISPR/Cas9 off‐target effects and biological variations in mammalian genome editing experiments. J. Biotechnol. 284: 91–101.
41 Kodama, M., Kodama, T., Newberg, J.Y. et al. (2017). in vivo loss‐of‐function screens identify KPNB1 as a new druggable oncogene in epithelial ovarian cancer. Proc. Natl. Acad. Sci. U. S. A. 114: E7301–E7310.
42 Labun, K., Montague, T.G., Krause, M. et al. (2019). CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing. Nucleic Acids Res. 47: W171–W174.
43 Lafleur, M.W., Nguyen, T.H., Coxe, M.A. et al. (2019). A CRISPR‐Cas9 delivery system for in vivo screening of genes in the immune system. Nat. Commun. 10: 1668.
44 Le Sage, C., Lawo, S., and Cross, B.C.S. (2020). CRISPR: a screener's guide. SLAS Discov 25: 233–240.
45 Li, K., Liu, Y., Cao, H. et al. (2020). Interrogation of enhancer function by enhancer‐targeting CRISPR epigenetic editing. Nat. Commun. 11: 485.
46 Liang, X., Potter, J., Kumar, S. et al. (2015). Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection. J. Biotechnol. 208: 44–53.
47 Lin, Y., Cradick, T.J., Brown, M.T. et al. (2014). CRISPR/Cas9 systems have off‐target activity with insertions or deletions between target DNA and guide RNA sequences. Nucleic Acids Res. 42: 7473–7485.
48 Liu, G., Zhang, Y., and Zhang, T. (2020). Computational approaches for effective CRISPR guide RNA design and evaluation. Comput. Struct. Biotechnol. J. 18: 35–44.
49 Lu, Q., Livi, G.P., Modha, S. et al. (2017). Applications of CRISPR genome editing technology in drug target identification and validation. Expert Opin. Drug Discovery 12: 541–552.
50 Manguso, R.T., Pope, H.W., Zimmer, M.D. et al. (2017). in vivo CRISPR screening identifies Ptpn2 as a cancer immunotherapy target. Nature 547: 413–418.
51 Mans, R., Van Rossum, H.M., Wijsman, M. et al. (2015). CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae. FEMS Yeast Res. 15.
52 Martin‐Laffon, J., Kuntz, M., and Ricroch, A.E. (2019). Worldwide CRISPR patent landscape shows strong geographical biases. Nat. Biotechnol. 37: 613–620.
53 Martufi, M., Good, R.B., Rapiteanu, R. et al. (2019). Single‐step, high‐efficiency CRISPR‐Cas9 genome editing in primary human disease‐derived fibroblasts. CRISPR J 2: 31–40.
54 Metzakopian, E., Strong, A., Iyer, V. et al. (2017). Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries. Sci. Rep. 7: 2244.
55 Mou, H., Smith, J.L., Peng, L. et al. (2017). CRISPR/Cas9‐mediated genome editing induces exon skipping by alternative splicing or exon deletion. Genome Biol. 18: 108.
56 Munoz, D.M., Cassiani, P.J., Li, L. et al. (2016). CRISPR screens provide a comprehensive assessment of cancer vulnerabilities but generate false‐positive hits for highly amplified genomic regions. Cancer Discov. 6: 900–913.
57 Najm, F.J., Strand, C., Donovan, K.F. et al. (2018). Orthologous CRISPR‐Cas9 enzymes for combinatorial genetic screens. Nat. Biotechnol. 36: 179–189.
58 Oughtred, R., Stark, C., Breitkreutz, B.J. et al. (2019). The BioGRID interaction database: 2019 update. Nucleic Acids Res. 47: D529–D541.
59 Pattanayak, V., Lin, S., Guilinger, J.P. et al. (2013). High‐throughput profiling of off‐target DNA cleavage reveals RNA‐programmed Cas9 nuclease specificity. Nat. Biotechnol. 31: 839–843.
60 Pichler, F.B. and Turner, S.J. (2007). The power and pitfalls of outsourcing. Nat. Biotechnol. 25: 1093–1096.
61 Popp, M.W. and Maquat, L.E. (2016). Leveraging rules of nonsense‐mediated mRNA decay for genome engineering and personalized medicine. Cell 165: 1319–1322.
62 Qiu, P., Shandilya, H., D'alessio, J.M. et al. (2004). Mutation detection using Surveyor nuclease. BioTechniques 36: 702–707.
63 Ran, F.A., Cong, L., Yan, W.X. et al. (2015). in vivo genome editing using Staphylococcus aureus Cas9. Nature 520: 186–191.
64 Rosenbluh, J., Xu, H., Harrington, W. et al. (2017). Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression. Nat. Commun. 8: 15403.
65 Safari, F., Zare, K., Negahdaripour, M. et al. (2019). CRISPR Cpf1 proteins: structure, function and implications for genome editing. Cell Biosci. 9: 36.
66 Sanson, K.R., Hanna, R.E., Hegde, M. et al. (2018). Optimized libraries for CRISPR‐Cas9 genetic screens with multiple modalities. Nat. Commun. 9: 5416.
67 Sanson, K.R., Deweirdt, P.C., Sangree, A.K. et al. (2020). Optimization of AsCas12a for combinatorial genetic screens in human cells. bioRxiv https://www.biorxiv.org/content/10.1101/747170v1.
68 Seki, A. and Rutz, S. (2018). Optimized RNP transfection for highly efficient CRISPR/Cas9‐mediated gene knockout in primary T cells. J. Exp. Med. 215: 985–997.
69 Sharpe, J.J. and Cooper, T.A. (2017). Unexpected consequences: exon skipping caused by CRISPR‐generated mutations. Genome Biol. 18: 109.
70 Shifrut, E., Carnevale, J., Tobin, V. et al. (2018). Genome‐wide CRISPR screens in primary human T cells reveal key regulators of immune function. Cell 175: 1958–1971. e15.
71 Slaymaker, I.M., Gao, L., Zetsche, B. et al. (2016). Rationally engineered Cas9 nucleases with improved specificity. Science 351: 84–88.
72 Smits, A.H., Ziebell, F., Joberty, G. et al. (2019). Biological plasticity rescues target activity in CRISPR knock outs. Nat. Methods 16: 1087–1093.
73 Song,
