suggesting a mechanism for their traditionally recognized antidiabetic properties (Kellogg et al. 2014).
Table 3.1 Portfolio of mobile biodiscovery training modules used in workshops with both elders and youth in participating native communities.
| Module ID | Approximate workshop time required to conduct the bioassays/procedures | How long before results can be evaluated | Relevant‐disease targets |
|---|---|---|---|
| Field collection* | 2–3 hours | n/a | n/a |
| Plant material extraction | 1–2 hours | n/a | n/a |
| Antibacterial assay | 2–3 hours | 24–48 hours | Bacterial pathogens |
| Antioxidant assay | 1 hour | Immediate | General health |
| Glucosidase and glucosidase inhibitor assays | 2 hours | Immediate | Metabolic syndrome type 2 diabetes |
| α‐amylase inhibition | 1–2 hours | Immediate | Metabolic syndrome type 2 diabetes |
| Anthocyanin detection | 1 hour | Immediate | General health |
| Protease and protease inhibitor assay | 30 minutes–1 hour | 10 minutes | Digestive diseases Viral pathogens |
| Planaria lethality assay | 1–2 hours | 8–24 hours | Parasitic worms |
| Planaria regeneration assay | 1–2 hours | 5 days | Parasitic worms |
| Nematode lethality assay | 1 hour | 4 hours | Parasitic worms |
* Field identification of medicinal plants and sample collection are conducted in concert with local elders from the community.
Figure 3.2 Alaska Native youth engage in a workshop featuring simple mobile biodiscovery assays during a 2019 fish camp on the Yukon River in Alaska.
Source: Mary Ann Lila.
Crude extracts from the same six Alaskan seaweeds were assayed for inhibition against five common inflammatory markers (COX2, iNOS, TNFα, IL‐10, and MCP‐1). While red and green seaweed species exhibited some activity, the brown seaweeds demonstrated significant anti‐inflammatory capacity against all five markers. Subfractionation of the brown seaweed F. distichus revealed modulation in a dose‐dependent manner by some fractions of lipid metabolism gene expression in an adipocyte model. Multiple anti‐inflammatory properties in both adipose and macrophage cells were revealed, and two natural product classes (long chain mono‐ and polyunsaturated fatty acids and phlorotannin oligomers) were linked to these biomarkers (Kellogg et al. 2015). The multifunctional activities of edible seaweeds towards inhibition of the cardiometabolic risk factors associated with metabolic syndrome were linked to high phenolic content and coincident angiotensin converting enzyme I inhibitor activity and anti‐inflammatory activities (Rico et al. 2018).
3.5.2 Isolating Phytoactive Principles from the Mediterranean Region
The mobile bioassay approach featured prominently in a joint ENPI CBCMED (European Neighbourhood and Partnership Instrument [ENPI] Cross‐border Cooperation [CBC] Mediterranean [MED] Program) grant (funded by the European Union) which linked scientists from Israel and the Palestinian Authority, along with Greece and Spain. The program conducted an efficient yet comprehensive survey of medicinal plants in the region, and identified primary leads with high potency bioactivities that held promise for development and further commercialization (Joseph et al. 2014). The mobile biodiscovery assays enabled rapid screening over a wide diversity of climate zones and habitats, and cataloguing of plant materials that were used in traditional medicine, but had not been assayed previously (Joseph et al. 2014; Ali‐Shtayeh et al. 2015). Natural areas in the wild were screened systematically over multiple territories across Palestine’s West Bank and Israel. Both in Palestine (Ali‐Shtayeh et al. 2015) and in Israel (Joseph et al. 2014), the research teams confirmed that the plants growing in the most hostile wild environments demonstrated more potent bioactive properties than plants in moderate environments. Regions with high solar radiation levels hosted plant materials with the highest recorded antioxidant levels and the most concentrated and diverse profiles of flavonoids and phenylpropanoids. Polyphenol‐rich plants were particularly identified as active against carbohydrate hydrolyzing enzymes (important in diabetes management). Commercial α‐glucosidase‐inhibiting drugs in general have been associated with gastrointestinal side effects, whereas the polyphenolic phytoactives were identified as alternatives that did not cause side reactions (Ali‐Shtayeh et al. 2015).
Figure 3.3 (a) Fucus distichus (bladder wrack), a traditionally used phlorotannin‐rich seaweed harvested in Whittier, AK. (b) Structural units of phlorotannins from the Alaskan brown algae F. distichus.
Source: (a) Mary Ann Lila.
3.5.3 Drug Discovery in Cooperation with Traditional Healers in Botswana
In Botswana, the population at large, both in rural areas and in towns, subscribes to a mixture of traditional medicine and Western medicine. To the chagrin of traditional healers, however, the traditional practices are slowly falling out of fashion, and are not regarded in as high esteem as Western medicine, especially among younger citizens. In an attempt to bridge this gap, a series of mobile discovery workshops was conducted in two regions of the country for traditional healers and other community members, who were invited to provide their own indigenous medicinal plants for use in the bioassay screens (Andrae‐Marobela et al. 2012). The mobile biodiscovery approach was used to establish an indigenous knowledge‐guided drug‐discovery platform. Because opportunistic infections such as tuberculosis and sexually transmitted infections are relatively high in Botswana, mobile screens were specifically tailored
