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review questions
1. What is biotechnology?
2. Distinguish between traditional biotechnology and molecular biotechnology.
3. What are the shortcomings of the “mutation and selection” method for developing enhanced organisms for commercial purposes?
4. Why was the work reported by Cohen and Boyer and their coworkers in 1973 considered important?
5. How did recombinant DNA technology enable the production of human insulin?
6. What are some of the problems that molecular biotechnology has the potential to solve?
7. Discuss the statement “Molecular biotechnology is a diverse science.”
8. Discuss some of the social concerns that have been raised about molecular biotechnology.
9. Go to your favorite news website and conduct a search with the word “biotechnology.” Describe and discuss three recent biotechnology news stories.
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Fundamental Technologies
Preparation of DNA for Cloning
Insertion of Target DNA into a Plasmid Vector
Transformation and Selection of Cloned DNA in a Bacterial Host
Genome Engineering Using CRISPR Technology
Assembling Oligonucleotides into Genes
Sequencing Using Reversible Chain Terminators
Sequencing by Single Molecule Synthesis
Preparation of Genomic DNA Sequencing Libraries
High-Throughput Next-Generation Sequencing Strategies
Molecular Cloning
MOLECULAR BIOTECHNOLOGY USES A variety of techniques for isolating genes and transferring them from one organism to another. At the root of these technologies is the ability to join a sequence of deoxyribonucleic acid (DNA)
