FIFTH EDITION
molecular biotechnology
Principles and Applications of Recombinant DNA
FIFTH EDITION
molecular biotechnology
Principles and Applications of Recombinant DNA
Bernard R. Glick
Department of Biology, University of Waterloo
Waterloo, Ontario, Canada
Cheryl L. Patten
Biology Department, University of New Brunswick,
Fredericton, New Brunswick, Canada
WASHINGTON, DC
The two of us thank our life partners, Marcia Glick and Patrick Patten, for the enormous support and encouragement that they have provided throughout this endeavor
B. R. Glick
C. L. Patten
Copyright © 2017 American Society for Microbiology. All rights reserved. No part of this publication may be reproduced or transmitted in whole or in part or reused in any form or by any means, electronic or mechanical, including photocopying and recording, or by any information storage and retrieval system, without permission in writing from the publisher.
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Library of Congress Cataloging-in-Publication Data
Names: Glick, Bernard R., author. | Patten, Cheryl L., author.
Title: Molecular biotechnology : principles and applications of recombinant DNA / Bernard R. Glick, Cheryl L. Patten.
Description: 5th edition. | Washington, DC : ASM Press, [2017]
Identifiers: LCCN 2017011321 | ISBN 9781555819361 (hardcover) | ISBN 9781683673101 (ebook)
Subjects: LCSH: Biotechnology. | Genetic engineering. | Molecular biology.
Classification: LCC TP248.2 .G58 2017 | DDC 660.6—dc23
LC record available at https://lccn.loc.gov/2017011321
All Rights Reserved
Printed in Canada
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doi: 10.1128/9781555819378
Cover illustration: Terese Winslow, CMI
Cover and interior design: Susan Brown Schmidler
Illustrations: Patrick Lane, ScEYEnce Studios
Cover Photo Credits
Plant - © KYTan/Shutterstock.com
Cells - © Jurlk Peter/Shutterstock.com
Pills - © RidvanArda/Shutterstock.com
Copper Bubbles - © tucsonazrealestateblog.com
Contents
1 The Development of Molecular Biotechnology
Emergence of Molecular Biotechnology
Commercialization of Molecular Biotechnology
Preparation of DNA for Cloning
Insertion of Target DNA into a Plasmid Vector
Transformation and Selection of Cloned DNA in a Bacterial Host
