Molecular Biotechnology. Bernard R. Glick

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href="#ulink_4052cb07-b036-51bc-be68-dc12bfa4ade6">Recombinational Cloning

       Genomic Libraries

       Genome Engineering using CRISPR Technology

       Polymerase Chain Reaction

       Amplification of DNA by PCR

       Cloning PCR Products

       Quantitative PCR

       Chemical Synthesis of DNA

       Synthesis of Oligonucleotides

       Assembling Oligonucleotides into Genes

       Gene Synthesis by PCR

       DNA Sequencing Technologies

       Dideoxynucleotide Procedure

       Pyrosequencing

       Sequencing using Reversible Chain Terminators

       Sequencing by Single Molecule Synthesis

       Sequencing Whole Genomes

       Preparation of Genomic DNA Sequencing Libraries

       High-Throughput Next-Generation Sequencing Strategies

       Genome Sequence Assembly

       Sequencing Metagenomes

       Genomics

       Transcriptomics

       Proteomics

       Metabolomics

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       3 Production of Recombinant Proteins

       Protein Production in Prokaryotic Hosts

       Regulation of Transcription

       Increasing Translation Efficiency

       Increasing Protein Stability

       Increasing Protein Secretion

       Facilitating Protein Purification

       DNA Integration into the Host Chromosome

       Heterologous Protein Production in Eukaryotic Cells

       Posttranslational Modification of Eukaryotic Proteins

       General Features of Eukaryotic Expression Systems

       Yeast Expression Systems

       Baculovirus–Insect Cell Expression Systems

       Mammalian Cell Expression Systems

       Protein Engineering

       Directed Mutagenesis

       Random Mutagenesis

       Examples of Protein Engineering

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       4 Molecular Diagnostics

       Immunological

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