Molecular Biotechnology. Bernard R. Glick

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Marker Genes from Chloroplast DNA

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       11 Transgenic Plants

       Insect Resistance

       B. thuringiensis Insecticidal Toxin

       Increasing Expression of the B. thuringiensis Protoxin

       Other Strategies for Protecting Plants against Insects

       Preventing the Development of B. thuringiensis-Resistant Insects

       Targeting Aphids

       Virus Resistance

       Viral Coat Protein-Mediated Protection

       Protection by Expression of Other Genes

       Herbicide Resistance

       Fungus and Bacterium Resistance

       Salt and Drought Stress

       Fruit Ripening and Flower Wilting

       Modification of Plant Nutritional Content

       Amino Acids

       Lipids

       Vitamins

       Iron

       Gluten

       Modification of Food Plant Taste and Appearance

       Preventing Discoloration

       Starch

       Plants as Bioreactors

       Antibodies

       Poly(3-hydroxybutyric Acid)

       Eddible Vaccines

       Plant Yield

       Altering Lignin Content

       Increasing Oxygen Content

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       12 Transgenic Animals

       Transgenic Mice: Methodology

       DNA Microinjection Method

       Retroviral Vector Method

       Engineered Embryonic Stem Cell Method

       Conditional Gene Inactivation with the Cre–loxP Recombination System

       Genome Editing with the CRISPR-Cas System

       Gene Knockdown by RNA Interference

       Transgenic Mice: Applications

       Transgenic Disease Models: Alzheimer Disease

       Transgenic Mice as Test Systems

       Control of Transgene Expression

       Conditional Control of Cell Death

       Transgenic Livestock

       Cloning Livestock by Somatic Cell Nuclear Transfer

       Production of Pharmaceuticals

       Production of Donor Organs

       Disease Resistant Livestock

       Improving Milk Quality

       Improving Animal Production Traits

       Transgenic Poultry

       Transgenic Fish

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       13 Molecular Biotechnology and Society

       Development of Guidelines for Recombinant DNA Research

       Deliberate Release of Genetically Modified Microorganisms

       Environmental Concerns

       Regulations

       Regulation of Genetically Modified Foods

       Food Ingredients Produced by Genetically Engineered Microorganisms

       Genetically Modified Crops

       Genetically Engineered Livestock

       Societal Concerns About Genetically Modified Foods

       Alteration of Nutritional Content of Food

       Potential for Introducing Toxins or Allergens into Food

       Potential for Transferring Transgenes from Food to Humans or Intestinal Microorganisms

       Controversy About the Labeling of Genetically Modified Foods

       Impact of Genetically Engineered Crops on Biodiversity

       Who Benefits from Production of Genetically Modified Foods?

       Environmental Benefits of Genetically Modified Crops

       How do Views about Genetically Engineered Organisms Impact Trade?

       Regulation and Safety of Medical Products of Biotechnology

       New Biological Drugs

       Genetic and Genomic Testing

       Economic Issues

       Patenting Biotechnology

       Patenting

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