Foot-and-Mouth Virus
Streptococcus
Delivery
Peptide Vaccines
Malaria
Genetic Immunization: DNA Vaccines
Delivery
Cancer
Dental Caries
Attenuated Vaccines
Herpes Simplex Virus
Cholera
Salmonella Species
Leishmania Species
Vector Vaccines
Vaccines Directed against Viruses
Vaccines Directed against Bacteria
Bacteria as Antigen Delivery Systems
Monoclonal Antibody Passive Immunity
Influenza Virus
SUMMARY
REFERENCES
REVIEW QUESTIONS
8 Industrial and Environmental Uses of Recombinant Microorganisms
Restriction Endonucleases
Small Biological Molecules
L-Ascorbic Acid
Indigo
Amino Acids
Lycopene
Antibiotics
Biopolymers
Microbial Degradation of Xenobiotics
Genetic Engineering of Biodegradative Pathways
Utilization of Starch and Sugars
Commercial Production of Fructose and Alcohol
Increasing Alcohol Production
Improving Fructose Production
Utilization of Cellulose and Hemicellulose
Lignocellulosics
Cellulase Genes
Direct Conversion of Biomass to Ethanol
Zymomonas mobilis
Lipids from Cyanobacteria
Hydrogen Production
SUMMARY
REFERENCES
REVIEW QUESTIONS
9 Large-Scale Production of Proteins from Recombinant Microorganisms
Principles of Microbial Growth
Batch Fermentation
Fed-Batch Fermentation
Continuous Fermentation
Maximizing The Efficiency of The Fermentation Process
High-Density Cell Cultures
Increasing Plasmid Stability
Quiescent E. Coli Cells
Protein Secretion
Reducing Acetate
Bioreactors
Typical Large-Scale Fermentation Systems
Two-Stage Fermentation in Tandem Airlift Reactors
Two-Stage Fermentation in a Single Stirred-Tank Reactor
Batch versus Fed-Batch Fermentation
Harvesting Microbial Cells
Disrupting Microbial Cells
Downstream Processing
Protein Solubilization
Utilizing an Immobilized Enzyme
Magnetic Separation of Proteins
Large-Scale Production of Plasmid DNA
SUMMARY
REFERENCES
REVIEW QUESTIONS
10 Genetic Engineering of Plants: Methodology
Plant Transformation with the Ti Plasmid of A. Tumefaciens
Ti Plasmid-Derived Vector Systems
Microprojectile Bombardment
Chloroplast Engineering
Very High Level Protein Expression
Use of Reporter Genes in Transformed Plant Cells
Manipulation of Gene Expression in Plants
Transient Gene Expression
Plant Promoters
Targeted Gene Editing
Facilitating Protein Purification
Protein Glycosylation
Production of Marker-Free Transgenic Plants
Removing Marker Genes from Nuclear DNA
