Secondary Metabolites of Medicinal Plants. Bharat Singh
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2.10.2 Culture Conditions
The leaf as explant was cultured on MS, B5, and Schenk & Hidebrandt (SH) media supplemented with different combination of different NAA with BA and Kin for shoot induction. Maximum shoot induction rate observed in MS medium supplemented with NAA and BA in leaf explants (Sathyaprabha et al. 2010; Lakshmi and Padmaja 2011). Similarly maximum rooting was obtained on B5 medium supplemented with NAA and roots developed within 25 days after inoculation (Abdi et al. 2013). The exposure of dark period enhanced the production of 3,4-dihydro-2,4,8,9-tetrahydroxy-6-methyl-1(2H)-anthracenone-4-O-β-D-glucopyranoside and 3,4-dihydro-2-methoxy-4,8,9-trihydroxy-6-methyl-1(2H)-anthracenone-4-O-β-D-glucopyranoside in cell cultures of A. barbadensis (Yagi et al. 1998).
This is a medicinal plant in which useful secondary metabolites are found sufficiently. The secondary products including as aloe emodin and chrysophanol synthesized via a plant-specific type III polyketide biosynthesis pathway. Authors also examined the effect of endogenous elicitors on the type III polyketide biosynthesis pathway and identified the metabolic changes induced in elicitor-treated