Genome Engineering for Crop Improvement. Группа авторов

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target="_blank" rel="nofollow" href="https://doi.org/10.7554/eLife.45973">https://doi.org/10.7554/eLife.45973.

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      Note

      1 # Equal Contribution

       Katarina Vogel‐Mikuš1,2, Paula Pongrac1,2, Ivan Kreft3, Primož Pelicon2, Primož Vavpetič2, Boštjan Jenčič2, Johannes Teun van Elteren4, Peter Kump2, Sudhir P. Singh5, and Marjana Regvar1

       1 Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia

       2 Jozef Stefan Institute, Ljubljana, Slovenia

       3 Nutrition Institute, Ljubljana, Slovenia

       4 National Institute of Chemistry, Ljubljana, Slovenia

       5 Center of Innovative and Applied Bioprocessing (DBT‐CIAB), Mohali, Punjab, India

      CHAPTER MENU

        2.1 Introduction

        2.2 Exploring Nutrient Distribution in Grain 2.2.1 Matrix Assisted Laser/Desorption/Ionization 2.2.2 Secondary Ion Mass Spectroscopy 2.2.3 Fourier Transform Infrared Spectroscopy

        2.3 Exploring the Mineral Distribution in Grain 2.3.1 Nuclear Microprobe 2.3.2 Synchrotron Radiation X‐Ray Florescence Spectrometry 2.3.3 Laser Ablation‐Inductively Coupled Plasma Mass Spectrometry 2.3.4 Nano Secondary Ion Mass Spectrometry 2.3.5 Sample Preparation

        2.4 Prospect

      Plants provide us with essential energy‐rich compounds, minerals, vitamins, amino and fatty acids, and a wealth of health‐promoting compounds (Eckardt 2011). The cultivation of new, high‐yielding varieties and the ever‐increasing use of mineral fertilizers and pesticides has led to a significant increase in crop yields. However, the quality of edible plants related to human nutrition did not receive proper attention until the 1990s, when an increasing number of reports started to appear on inadequate diet and unbalanced nutrition in vulnerable, low‐income populations in many countries, leading to poor health, low productivity and an increase in chronic diseases (Eckardt 2011).

      This chapter will focus on cereals and pseudo‐cereal plants that produce starchy grains since they are part

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