Need for Reliable and Plentiful Information about Disease Pathology
Selected Readings
Questions
Solving Problems in Bacterial Pathogenesis
CHAPTER 9 Identification of Virulence Factors: Molecular Approaches for Bacterial Factors
Finding a Needle in a Haystack
Biochemical Approaches
Isolation and Purification of Toxic Factors
Molecular Genetic Approaches
Screening Using Recombinant Genes
Reporter Fusions
Mutagenesis Screening
Genome-wide Sequencing Approaches for Identifying Virulence Genes
Tn-Seq Technology to Identify In Vivo-Expressed Genes
RNA-Seq Technology to Identify In Vivo-Expressed Genes
Comparative Genomic Sequence Analysis for Identifying Virulence Genes
Proteomics Approaches for Identifying Virulence Factors
Protein Microarrays (Proteoarrays)
In Vivo-Induced Antigen Technology (IVIAT)
The Importance of Understanding Bacterial Physiology
Selected Readings
Questions
Solving Problems in Bacterial Pathogenesis
CHAPTER 10 Identification of Virulence Factors: Molecular Approaches for Host Factors
Comparative Approaches to Identify Host Factors Required for Infection
Transgenic Animal Models
In vivo Imaging of Animals during Infection
Systems Genetics: Comparative Genomics of the Host Response
Screening Approaches to Identify Host Factors Required for Infection
Genome-Wide Screening
Host Response Profiling to Identify Host Factors Required for Infection
Transcriptomics
Proteomics
Metabolomics
The Promise and the Caution
Selected Readings
Questions
Solving Problems in Bacterial Pathogenesis
CHAPTER 11 Bacterial Strategies for Colonization and Survival in the Host
What Does Not Kill You Makes You Stronger—Or, a Better Pathogen
Preinfection
Survival in the External Environment
Biofilms
Motility and Chemotaxis
Colonization of Host Surfaces
Penetrating Intact Skin
Penetrating the Mucin Layer
Evading the Host’s Innate Immunity
Nutrient and Iron Acquisition Mechanisms
Adherence
Evading the Host Immune Response
Avoiding Complement and Phagocytosis
Invasion and Uptake by Host Cells
Surviving Phagocytosis
Cell-to-Cell Spread
Tissue Penetration and Dissemination
Beyond Virulence Factors
Selected Readings
Questions
Solving Problems in Bacterial Pathogenesis
Special Global Perspective Problem: Integrating Concepts in Pathogenesis
CHAPTER 12 Toxins and Other Toxic Virulence Factors
Bacterial Toxins
Transparent Mechanisms, Exciting Applications, Mysterious Purposes
Toxin Characteristics and Nomenclature
Nonprotein Toxins
Peptide and Protein Exotoxins
Toxic Effector Proteins of Specialized Secretion Systems
Examples of Toxin-Mediated Diseases
Diphtheria Toxin
Clostridial Neurotoxins
Cholera Toxin
Toxin-Based Therapeutics and Research Tools
Immunotoxins
Selected Readings
Questions
Solving Problems in Bacterial Pathogenesis
CHAPTER 13 Delivery of Virulence Factors
Bacterial Secretion Systems and Virulence
Common Secretory Systems
The General Secretory (Sec) System
