Equine Reproductive Procedures. Группа авторов
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39 Chapter 50Figure 50.1 A sterile controlled flushing set is spiked into a pre‐warmed ba...Figure 50.2 Positioning the TVA handle encasing the ultrasound probe per vag...Figure 50.3 (a) Lab equipment (dissecting microscope with heated stage, 150 ...Figure 50.4 Pre‐warmed 75 μm embryo filtration cup with contents of immature...Figure 50.5 (a) Image of maturing oocyte recovered from a hormone‐stimulated...
40 Chapter 51Figure 51.1 Two examples of isothermic containers utilized for shipment of o...
41 Chapter 52Figure 52.1 Equine oocyte held in place with suction from a holding pipette ...
42 Chapter 53Figure 53.1 Grasping the dorsal commissure of the vulva in preparation for s...Figure 53.2 Placement of skin staples in the vulvar labia for staple vulvopl...Figure 53.3 Finished temporary vulvoplasty.Figure 53.4 Trimming mucosa from the inner labia.Figure 53.5 Suturing the labia together.Figure 53.6 Placement of fingers for the removal of the vulvoplasty.Figure 53.7 Cutting the co‐joined mucosa to remove the vulvoplasty.
43 Chapter 54Figure 54.1 Culture of the central clitoral sinus using an ultrafine culture...Figure 54.2 Culture of the clitoral fossa.Figure 54.3 Flushing the central clitoral fossa using a curved tip syringe....Figure 54.4 Packing the clitoris with 0.2% nitrofurazone antibiotic ointment...Figure 54.5 Culture locations on the distal penis: urethral sinus (thin arro...
44 Chapter 55Figure 55.1 Mare in box stall with a fluorescent light overhead.Figure 55.2 Mare wearing a mask emitting a blue light into one eye.
45 Chapter 57Figure 57.1 Insertion of an Ovuplant™ implant (arrow) in the vulvar mucosa....
46 Chapter 59Figure 59.1 Unsterilized 35 mm glass ball.Figure 59.2 Ultrasound image of an intrauterine glass ball. The arrow points...Figure 59.3 Disassembled iUPODTM applicator: outer tube (A), applicator plun...Figure 59.4 Disassembled iUPODTM magnetic retriever and applicator: applicat...Figure 59.5 Ultrasound image of an iUPODTM (arrow) in the uterus. Note the a...Figure 59.6 Ultrasound image of a 16‐day embryonic vesicle (black arrow) adj...
47 Chapter 61Figure 61.1 Ovaries (small arrow) in a plastic bag are placed into the cente...
48 Chapter 62Figure 62.1 Non‐surgical embryo collection procedure.Figure 62.2 Embryo collection setup. The media bag is connected by “Y” tubin...Figure 62.3 Flush media passing through an embryo filter (open system).Figure 62.4 Flush media passing through an embryo filter (closed system).Figure 62.5 Straw (0.25 ml), connector and 1.0 ml syringe for handling small...Figure 62.6 Petri dish (35 mm) with holding medium (left side). Washing an e...
49 Chapter 63Figure 63.1 Morula stage embryo (grade 1). Note the thick zona pellucida and...Figure 63.2 Early blastocyst stage embryo (grade 1). Note the thinner zona p...Figure 63.3 Blastocyst stage embryo (grade 1). Note the thin zona pellucida,...Figure 63.4 Expanded blastocyst stage embryo (grade 1). The zona pellucida h...Figure 63.5 Unfertilized oocytes. Note the thick zona pellucida and lack of ...Figure 63.6 Grade 2 early blastocyst stage embryo. Note the extruded blastom...Figure 63.7 Measurement of embryo diameter using an eye piece micrometer.
50 Chapter 64Figure 64.1 Aspiration biopsy of an equine blastocyst embryo.
51 Chapter 65Figure 65.1 Left to right, 50 ml conical tube, 5 ml vial, and Parafilm® for ...Figure 65.2 Sealing the 5 ml vial with Parafilm®. Pull the Parafilm® so that...Figure 65.3 Placing the small 5 ml vial containing the embryo inside the lar...Figure 65.4 The 50 ml conical tube sealed with Parafilm® with a sealed 5 ml t...Figure 65.5 Loading the 50 ml conical tube into a passive cooling system for...
52 Chapter 66Figure 66.1 Petri dish prepared for vitrification of an embryo. VS1, solutio...Figure 66.2 Thermos with a cane holding a goblet with the top suspended abov...Figure 66.3 Straw placed into a goblet that is suspended above liquid nitrog...Figure 66.4 Embryo plunged into liquid nitrogen.
53 Chapter 67Figure 67.1 Embryo in a column of holding medium (large arrow) inside a 0.25...
54 Chapter 68Figure 68.1 A filly successfully born from an in vitro produced embryo trans...Figure 68.2 A mature oocyte from an autotransfer donor undergoing the ICSI p...Figure 68.3 A 14‐day embryonic vesicle (yellow dotted circle) (a) following ...
55 Chapter 69Figure 69.1 Palpation of a uterine specimen demonstrating the technique for ...
56 Chapter 70Figure 70.1 Ultrasonographic image of a 12‐day pregnancy.Figure 70.2 Ultrasonographic image of a 16‐day pregnancy.Figure 70.3 Ultrasonographic image of a 25‐day pregnancy.Figure 70.4 Ultrasonographic image of a 35‐day pregnancy.Figure 70.5 Ultrasonographic image of twin pregnancies, with the embryonic v...Figure 70.6 Ultrasonographic image of twin pregnancies, with the embryos tou...Figure 70.7 Ultrasonographic image of a 25‐day pregnancy examination showing...Figure 70.8 Ultrasonographic image of a 25‐day pregnancy (arrow) and adjacen...Figure 70.9 Ultrasonographic image of an empty trophoblastic vesicle 28 days...Figure 70.10 Ultrasonographic image of multiple supplementary (accessory) co...
57 Chapter 71Figure 71.1 Cervix of a pregnant mare viewed through a speculum (day 45 of g...
58 Chapter 72Figure 72.1 Location and appearance of the genital tubercle (arrow) in a mal...Figure 72.2 Location and appearance of the genital tubercle (arrow) in a fem...Figure 72.3 Directions of ultrasound imaging for fetal sexing (planes I, II,...Figure 72.4 Genital tubercle (arrows) of a 68‐day‐old male equine fetus in p...Figure 72.5 (a–c) Genital tubercle (arrows) of a 65‐day‐old female equine fe...Figure 72.6 Ultrasound image of an equine fetus in plane III at day 100 of g...Figure 72.7 Ultrasound image of a male equine fetus in plane II at 95 days o...Figure 72.8 Female equine fetus at 112 days of gestation showing the hyperec...Figure 72.9 Ultrasound image of a female equine fetus at 125 days of gestati...Figure 72.10 Ultrasound image of a mammary gland (red arrow) and teats (blue...Figure 72.11 Ultrasound image of the mammary gland of a female equine fetus ...Figure 72.12 Glans penis (blue arrow) within the prepuce of a male equine fe...Figure 72.13 Gonads (testes) of a male equine fetus at 130 days of gestation...Figure 72.14 Gonads (ovaries) of a female equine fetus.
59 Chapter 73Figure 73.1 Portable pump sprayer for application of alcohol prior to ultras...Figure 73.2 Transabdominal ultrasonography of a late‐term pregnant mare.Figure 73.3 Transrectal ultrasonography of placental edema in a near‐term no...
60 Chapter 74Figure 74.1 Color Doppler ultrasound image demonstrating the uterine branch ...Figure 74.2 Ultrasound measurement of the combined thickness of the uterus a...Figure 74.3 Average combined thickness of the uterus and placenta in normal ...Figure 74.4 Ultrasound image of a placenta exhibiting increased thickness an...Figure 74.5 Ultrasound image showing separation (arrows) of the chorioallant...
61 Chapter 75Figure 75.1 Milk secretions in a mare with precocious mammary development.Figure 75.2 A late‐term mare with purulent vaginal discharge.Figure 75.3 A late‐term mare with purulent discharge in the ventral tail hai...Figure 75.4 Measurement of the combined thickness of the uterus and placenta...Figure 75.5 An area of separation of the chorioallantois from the uterine wa...
62 Chapter 76Figure 76.1 Pulsed Doppler used to obtain the heart rate during a transabdom...Figure 76.2 Color Doppler image of the cranial cervix and caudal uterus obta...Figure 76.3 (a) Upper transabdominal image demonstrating potential placental...
63 Chapter 77Figure 77.1 A graph of the maximum height (mm) of the embryonic vesicle vers...Figure 77.2 Aortic root diameter measurement of 22 mm in a mare 10 months’ p...Figure 77.3 Per rectum measurement of fetal eye diameter showing a vertical ...
64 Chapter 78Figure 78.1